1) Open ImageJ (Fiji)
2) Load image
3) Select "rectangle" tool
4) Draw ROI for DMS (right side first), then press "t" to add to ROI manager.
5) Make sure that in ROI manager, the options "Show All" and "Labels" are checked.
6) Draw ROI for DLS (right side first), then press "t" to add to ROI manager.
7) Draw ROI for DMS then DLS for left side as well if needed.
8) Make sure all ROIs in ROI manager are selected, then "more" -> "save" -> save as "MouseName**_SlideName**_crop_ROI". File extension should be .roi for single ROI, and .zip for multiple ROIs.
